parental strains s aureus atcc 12600  (ATCC)

Journal: Frontiers in Immunology

Article Title: A Strong Synergy Between the Thiopeptide Bacteriocin Micrococcin P1 and Rifampicin Against MRSA in a Murine Skin Infection Model

doi: 10.3389/fimmu.2021.676534

Figure Lengend Snippet: Synergy assessment between MP1 and a panel of antibiotics against MRSA Xen31.

Article Snippet: For topical use in mice the final mixture in APO Base 30 cream contained 10 μg/ml MP1 and 150 μg/ml rifampicin which were about 100 times higher than their MIC values recorded in the checkerboard assay, since MRSA Xen31 parental strain S. aureus ATCC 33591 is known to produce biofilms on surfaces within 24h ( ) and staphylococcal biofilms are 10- to 1000-fold more resistant to antimicrobials compared to planktonic cells ( ).

Techniques:

Journal: Frontiers in Immunology

Article Title: A Strong Synergy Between the Thiopeptide Bacteriocin Micrococcin P1 and Rifampicin Against MRSA in a Murine Skin Infection Model

doi: 10.3389/fimmu.2021.676534

Figure Lengend Snippet: Assessment of the antimicrobial activity of MP1(10 µg/ml), rifampicin (150 µg/ml) and the combination (MP1, 10µg/ml + rifampicin, 150µg/ml) in APO base cream 30%. Cream with not addition was included as negative control. The activity was tested with softagar overlay assay using MRSA Xen31 as indicator strain.

Article Snippet: For topical use in mice the final mixture in APO Base 30 cream contained 10 μg/ml MP1 and 150 μg/ml rifampicin which were about 100 times higher than their MIC values recorded in the checkerboard assay, since MRSA Xen31 parental strain S. aureus ATCC 33591 is known to produce biofilms on surfaces within 24h ( ) and staphylococcal biofilms are 10- to 1000-fold more resistant to antimicrobials compared to planktonic cells ( ).

Techniques: Activity Assay, Cream, Negative Control, Overlay Assay

Journal: Frontiers in Immunology

Article Title: A Strong Synergy Between the Thiopeptide Bacteriocin Micrococcin P1 and Rifampicin Against MRSA in a Murine Skin Infection Model

doi: 10.3389/fimmu.2021.676534

Figure Lengend Snippet: Bioluminescence from mice skin infections during different treatments. Box plots of bioluminescent signals produced by MRSA Xen31 (in photons per second per square centimeter per steradian) in differently treated mouse groups. The days of treatment are indicated with arrows. (A) mice received daily treatments for nine days. (B) mice received daily treatments for four days before they were left untreated for the rest of the experiment. The area within each box represents the interquartile region (IQR), which comprises the second and third quartiles and describes the interval of values where the middle 50% of the observed data are distributed. The horizontal black line within each box represents the median value. The extent of the IQR (box height) express the degree of variability measured within the middle 50% of the observed data, with whiskers extending out at either side of the boxes marking the minimum and maximum observed values, as well as the variability outside the middle 50% of values (whisker length). Outliers are displayed as data that extend out of the whisker limit (1.5 times the IQR).

Article Snippet: For topical use in mice the final mixture in APO Base 30 cream contained 10 μg/ml MP1 and 150 μg/ml rifampicin which were about 100 times higher than their MIC values recorded in the checkerboard assay, since MRSA Xen31 parental strain S. aureus ATCC 33591 is known to produce biofilms on surfaces within 24h ( ) and staphylococcal biofilms are 10- to 1000-fold more resistant to antimicrobials compared to planktonic cells ( ).

Techniques: Produced, Whisker Assay

Journal: Frontiers in Immunology

Article Title: A Strong Synergy Between the Thiopeptide Bacteriocin Micrococcin P1 and Rifampicin Against MRSA in a Murine Skin Infection Model

doi: 10.3389/fimmu.2021.676534

Figure Lengend Snippet: (A) In vivo images of bioluminescent signals produced by MRSA Xen31(in photons/second/cm 2 /steradian) from the different mouse groups on the last day of the experiment; left column: with nine daily treatments; right column with four daily treatments. (B) Rifampicin and fucidin resistance develops during the treatment of mice. Rifampicin resistant cells (Rif-resistance) and fucidic acid resistant cells (Fucidic acid resistance) isolated from the wounds with strong bioluminescent signals were rechallenged and shown to be resistant to rifampicin (Rif) and fusidic acid (F.a.) but not to the MP1-rifampicin mixture. Wildtype MRSA Xen31 cells exposed to the MP1-ripamicin mixture, rifampicin and fusidic acid were sensitive to all three antimicrobials.

Article Snippet: For topical use in mice the final mixture in APO Base 30 cream contained 10 μg/ml MP1 and 150 μg/ml rifampicin which were about 100 times higher than their MIC values recorded in the checkerboard assay, since MRSA Xen31 parental strain S. aureus ATCC 33591 is known to produce biofilms on surfaces within 24h ( ) and staphylococcal biofilms are 10- to 1000-fold more resistant to antimicrobials compared to planktonic cells ( ).

Techniques: In Vivo, Produced, Isolation

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Evaluating the potential efficacy and limitations of a phage for joint antibiotic and phage therapy of Staphylococcus aureus infections

doi: 10.1073/pnas.2008007118

Figure Lengend Snippet: The joint action of bactericidal antibiotics and PYO Sa . Concentrations of the different antibiotics in μg/mL (RIF 0.02, OXA 3, CIP 0.5, VAN 8, DAP 64, KAN 46), corresponding to minimum bacteriocidal concentrations. ( A ) The ratio of the change in density of S. aureus after 24 h of exposure to antibiotics, antibiotics and phage, or phage alone. Hash red, the density of S. aureus recovered was below the detection limit, ∼10 2 per mL. ( B ) The ratio of the change in the density of PYO Sa after 24 h of confronting S. aureus Newman in combination with antibiotics or alone.

Article Snippet: Unless otherwise noted, all experiments were performed from derivatives of the parent strain S. aureus Newman (ATCC 25904).

Techniques: